275

19.8

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19.9

19.10

19.11

What Else Should Be Considered in a Sequence Analysis?

Importantly, now that you have found a hit, you should verify your result and back it up

with additional software searches, such as whether all the motifs or amino acid residues

are there that I need for the protein function (see also the PROSITE and AnDom exam­

ples). It is also important whether BLAST allows searching back, i.e. whether there is a hit

in the database again when I re-enter the sequence I hit with my query. Otherwise, it is

important that all the clues to my sequence (all the tests, all the data, all the other program

results) match each other and thus confirm the search. For example, a transcription factor

(according to the BLAST search, because it was most similar to it) should then also have

at least one DNA-binding domain in the domain composition, but also a protein localiza­

tion signal in the protein sequence, for example.

How Do I Perform a Simple Genome Annotation?

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(https://www.fruitfly.org/seq_tools/promoter.html

https://www.

prodoric.de/vfp/

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19  Tutorial: An Overview of Important Databases and Programs